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Quantitative detection of Secale cereale by real-time PCR amplification

LWT - Food Science and Technology, ISSN: 0023-6438, Vol: 37, Issue: 2, Page: 239-246
2004
  • 28
    Citations
  • 0
    Usage
  • 30
    Captures
  • 0
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    28
    • Citation Indexes
      27
    • Policy Citations
      1
      • 1
  • Captures
    30

Article Description

Wheat, barley, rye free diet is required to control coeliac disease and therefore adequate analytical tools are necessary to check gluten-free products. At the moment, official analytical methods are based on immunochemical assays for gliadin detection. However, DNA-based methods for detection of specific sequences have been extensively proposed as alternative to the protein-based ones, e.g. for transgenic contamination analysis. The aim of this work has been to develop an alternative quantification method for rye-content evaluation in raw materials and processed food based on real-time PCR approach using both SYBR green detection system and TaqMan fluorogenic probe.

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