MRTF-A signaling regulates the acquisition of the contractile phenotype in dedifferentiated chondrocytes
Matrix Biology, ISSN: 0945-053X, Vol: 62, Page: 3-14
2017
- 27Citations
- 34Captures
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Metrics Details
- Citations27
- Citation Indexes27
- 27
- CrossRef14
- Captures34
- Readers34
- 34
Article Description
Chondrocyte culture as a monolayer for cell number expansion results in dedifferentiation whereby expanded cells acquire contractile features and increased actin polymerization status. This study determined whether the actin polymerization based signaling pathway, myocardin-related transcription factor-a (MRTF-A) is involved in regulating this contractile phenotype. Serial passaging of chondrocytes in monolayer culture to passage 2 resulted in increased gene and protein expression of the contractile molecules alpha-smooth muscle actin, transgelin and vinculin compared to non-passaged, primary cells. This resulted in a functional change as passaged 2, but not primary, chondrocytes were capable of contracting type I collagen gels in a stress-relaxed contraction assay. These changes were associated with increased actin polymerization and MRTF-A nuclear localization. The involvement of actin was demonstrated by latrunculin B depolymerization of actin which reversed these changes. Alternatively cytochalasin D which activates MRTF-A increased gene and protein expression of α-smooth muscle actin, transgelin and vinculin, whereas CCG1423 which deactivates MRTF-A decreased these molecules. The involvement of MRTF-A signaling was confirmed by gene silencing of MRTF or its co-factor serum response factor. Knockdown experiments revealed downregulation of α-smooth muscle actin and transgelin gene and protein expression, and inhibition of gel contraction. These findings demonstrate that passaged chondrocytes acquire a contractile phenotype and that this change is modulated by the actin-MRTF-A-serum response factor signaling pathway.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0945053X16301561; http://dx.doi.org/10.1016/j.matbio.2016.10.004; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85006061987&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/27751947; https://linkinghub.elsevier.com/retrieve/pii/S0945053X16301561; https://dx.doi.org/10.1016/j.matbio.2016.10.004
Elsevier BV
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