PlumX Metrics
Embed PlumX Metrics

AMPK inhibits voltage-gated calcium channel-current in rat chromaffin cells

Molecular and Cellular Endocrinology, ISSN: 0303-7207, Vol: 591, Page: 112275
2024
  • 0
    Citations
  • 0
    Usage
  • 1
    Captures
  • 0
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

Article Description

Metabolic changes are critical in the regulation of Ca 2+ influx in central and peripheral neuroendocrine cells. To study the regulation of L-type Ca 2+ channels by AMPK we used biochemical reagents and ATP/glucose-concentration manipulations in rat chromaffin cells. AICAR and Compound-C, at low concentration, significantly induce changes in L-type Ca 2+ channel-current amplitude and voltage dependence. Remarkably, an overlasting decrease in the channel-current density can be induced by lowering the intracellular level of ATP. Accordingly, Ca 2+ channel-current density gradually diminishes by decreasing the extracellular glucose concentration. By using immunofluorescence, a decrease in the expression of Ca V 1.2 is observed while decreasing extracellular glucose, suggesting that AMPK reduces the number of functional Ca 2+ channels into the plasma membrane. Together, these results support for the first time the dependence of metabolic changes in the maintenance of Ca 2+ channel-current by AMPK. They reveal a key step in Ca 2+ influx in secretory cells.

Provide Feedback

Have ideas for a new metric? Would you like to see something else here?Let us know