A New Monoclonal Antibody Enables BAR Analysis of Subcellular Importin β1 Interactomes
Molecular & Cellular Proteomics, ISSN: 1535-9476, Vol: 21, Issue: 11, Page: 100418
2022
- 2Citations
- 7Captures
- 1Mentions
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- Citations2
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Most Recent News
Findings in the Area of Antibodies Reported from Weizmann Institute of Science (A New Monoclonal Antibody Enables Bar Analysis of Subcellular Importin Beta 1 Interactomes)
2023 JAN 27 (NewsRx) -- By a News Reporter-Staff News Editor at Middle East Daily -- Research findings on Proteins - Antibodies are discussed in
Article Description
Importin β1 (KPNB1) is a nucleocytoplasmic transport factor with critical roles in both cytoplasmic and nucleocytoplasmic transport, hence there is keen interest in the characterization of its subcellular interactomes. We found limited efficiency of BioID in the detection of importin complex cargos and therefore generated a highly specific and sensitive anti-KPNB1 monoclonal antibody to enable biotinylation by antibody recognition analysis of importin β1 interactomes. The monoclonal antibody recognizes an epitope comprising residues 301-320 of human KPBN1 and strikingly is highly specific for cytoplasmic KPNB1 in diverse applications, with little reaction with KPNB1 in the nucleus. Biotinylation by antibody recognition with this novel antibody revealed numerous new interactors of importin β1, expanding the KPNB1 interactome to cytoplasmic and signaling complexes that highlight potential new functions for the importins complex beyond nucleocytoplasmic transport. Data are available via ProteomeXchange with identifier PXD032728.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S1535947622002262; http://dx.doi.org/10.1016/j.mcpro.2022.100418; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85141330024&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/36180036; https://linkinghub.elsevier.com/retrieve/pii/S1535947622002262; https://dx.doi.org/10.1016/j.mcpro.2022.100418
Elsevier BV
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