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Molecular and phylogenetic characterization based on the complete genome of a virulent pathotype of Newcastle disease virus isolated in the 1970s in Brazil

Infection, Genetics and Evolution, ISSN: 1567-1348, Vol: 26, Page: 160-167
2014
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Single Step Detergent Assisted Extraction and Solubilization of the Recombinant Matrix Protein of Newcastle Disease Virus (NDV) and Development of ELISA for Detection of Anti-NDV Antibodies in Chickens.

Byline: Zahra Naz, Fouzia Ismat, Muhammad Saleem, Mazhar Iqbal, Aamir Shehzad and Moazur Rahman Key words Matrix protein, Newcastle disease virus, ELISA, Chicken, LDAO INTRODUCTION

Article Description

Newcastle disease (ND) is caused by the avian paramyxovirus type 1 (APMV-1) or Newcastle disease virus (NDV) that comprises a diverse group of viruses with a single-stranded, negative-sense RNA genome. ND is one of the most important diseases of chickens, because it severely affects poultry production worldwide. In the 1970s, outbreaks of virulent ND were recorded in Brazil, and the strain APMV-1/Chicken/Brazil/SJM/75 (SJM) of NDV was isolated. This strain was characterized as highly pathogenic for chickens but not pathogenic for other bird species. Here we present the complete genome of NDV strain SJM and investigate the phylogenetic relationships of this virus with other NDV strains in terms of genome and proteins composition, as well as characterizing its evolution process. The NDV strain SJM is categorized as a velogenic virus and the complete genome is 15,192 nucleotides in length, consisting of six genes in the order 3′-NP-P-M-F-HN-L-5′. The presence of the major pathogenic determinant of NDV strains ( 112 R-R-Q-K-R↓F 117 ) was identified in the Fusion protein of the NDV strain SJM. In addition, phylogenetic analysis classified the NDV strain SJM as a member of class II, genotype V, and indicates that this virus help us in the understanding of the evolutionary process of strains belonging to this genotype. This study contributes to the growing interest involving the characterization of NDV isolates to improve our current understanding about the epidemiology, surveillance and evolution of the pathogenic strains.

Bibliographic Details

Camila C. Fernandes; Alessandro M. Varani; Eliana G.M. Lemos; Vitor Fernandes O. de Miranda; Ketherson R. Silva; Filipe S. Fernando; Maria F.S. Montassier; Helio J. Montassier

Elsevier BV

Immunology and Microbiology; Agricultural and Biological Sciences; Biochemistry, Genetics and Molecular Biology; Medicine

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