Fourier transform infrared spectroscopy as a new tool for characterization of mollicutes
Journal of Microbiological Methods, ISSN: 0167-7012, Vol: 56, Issue: 1, Page: 73-82
2004
- 30Citations
- 23Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations30
- Citation Indexes30
- 30
- CrossRef21
- Captures23
- Readers23
- 23
Article Description
Fourier transform infrared (FT-IR) spectroscopy is a convenient physico-chemical technique to investigate various cell materials. Bacteria of class Mollicutes, identified by conventional methods, as Mycoplasma, Acholeplasma and Ureaplasma genera were characterized using this method. A data set of 74 independent experiments corresponding to fourteen reference strains of Mollicutes was examined by FT-IR spectroscopy to attempt a spectral characterization based on the biomolecular structures. In addition to the separation of Mollicutes within the lipidic region into five main clusters corresponding to the three phylogenetic groups tested, FT-IR spectroscopy allowed a fine discrimination between strains belonging to the same species by using selective spectral windows, particularly in the 1200–900 cm −1 saccharide range. The results obtained by FT-IR were in good agreement with both taxonomic and phylogenetic classifications of tested strains. Thus, this technique appears to be a useful tool and an accurate mean for a rapid characterization of Mollicutes observed in humans.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0167701203002732; http://dx.doi.org/10.1016/j.mimet.2003.09.020; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0347360158&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/14706752; https://linkinghub.elsevier.com/retrieve/pii/S0167701203002732; https://dx.doi.org/10.1016/j.mimet.2003.09.020
Elsevier BV
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