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Fourier transform infrared spectroscopy as a new tool for characterization of mollicutes

Journal of Microbiological Methods, ISSN: 0167-7012, Vol: 56, Issue: 1, Page: 73-82
2004
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Article Description

Fourier transform infrared (FT-IR) spectroscopy is a convenient physico-chemical technique to investigate various cell materials. Bacteria of class Mollicutes, identified by conventional methods, as Mycoplasma, Acholeplasma and Ureaplasma genera were characterized using this method. A data set of 74 independent experiments corresponding to fourteen reference strains of Mollicutes was examined by FT-IR spectroscopy to attempt a spectral characterization based on the biomolecular structures. In addition to the separation of Mollicutes within the lipidic region into five main clusters corresponding to the three phylogenetic groups tested, FT-IR spectroscopy allowed a fine discrimination between strains belonging to the same species by using selective spectral windows, particularly in the 1200–900 cm −1 saccharide range. The results obtained by FT-IR were in good agreement with both taxonomic and phylogenetic classifications of tested strains. Thus, this technique appears to be a useful tool and an accurate mean for a rapid characterization of Mollicutes observed in humans.

Bibliographic Details

Melin, Anne-Marie; Allery, Annie; Perromat, Annie; Bébéar, Christiane; Déléris, Gérard; de Barbeyrac, Bertille

Elsevier BV

Immunology and Microbiology; Biochemistry, Genetics and Molecular Biology; Medicine

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