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Reporting off-target effects of recombinant engineering using the pORTMAGE system

Journal of Microbiological Methods, ISSN: 0167-7012, Vol: 204, Page: 106627
2023
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New Engineering Study Results from North Carolina Agricultural and Technical State University Described (Reporting Off-target Effects of Recombinant Engineering Using the Portmage System)

2023 FEB 10 (NewsRx) -- By a News Reporter-Staff News Editor at Engineering Daily News -- Fresh data on Engineering are presented in a new

Article Description

pORTMAGE recombineering is a simple technique for incorporation of novel point mutations into bacterial genomes that eliminates off-target effects. Here we inserted point mutations into the cusS gene from Escherichia coli, then, using Illumina sequencing, report genetic variants in all mutant strains. Several off-site mutations were found at high frequency. Low frequency mutations also show high heterogeneity. This means that it is essential for studies to report all off-target effects and acknowledge the effect that this may have on resultant phenotypes.

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