Sequential ATP Hydrolysis by Cdc6 and ORC Directs Loading of the Mcm2-7 Helicase
Molecular Cell, ISSN: 1097-2765, Vol: 21, Issue: 1, Page: 29-39
2006
- 215Citations
- 231Captures
- 6Mentions
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations215
- Citation Indexes215
- 215
- CrossRef212
- Captures231
- Readers231
- 231
- Mentions6
- References6
- Wikipedia6
Article Description
Loading of the Mcm2-7 DNA replicative helicase onto origin-proximal DNA is a critical and tightly regulated event during the initiation of eukaryotic DNA replication. The resulting protein-DNA assembly is called the prereplicative complex (pre-RC), and its formation requires the origin recognition complex (ORC), Cdc6, Cdt1, and ATP. ATP hydrolysis by ORC is required for multiple rounds of Mcm2-7 loading. Here, we investigate the role of ATP hydrolysis by Cdc6 during pre-RC assembly. We find that Cdc6 is an ORC- and origin DNA-dependent ATPase that functions at a step preceding ATP hydrolysis by ORC. Inhibiting Cdc6 ATP hydrolysis stabilizes Cdt1 on origin DNA and prevents Mcm2-7 loading. In contrast, the initial association of Mcm2-7 with the other pre-RC components does not require ATP hydrolysis by Cdc6. Importantly, these coordinated yet distinct functions of ORC and Cdc6 ensure the correct temporal and spatial regulation of pre-RC formation.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S1097276505018113; http://dx.doi.org/10.1016/j.molcel.2005.11.023; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=29544435484&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/16387651; https://linkinghub.elsevier.com/retrieve/pii/S1097276505018113; https://dx.doi.org/10.1016/j.molcel.2005.11.023
Elsevier BV
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