How does dilution affect the conductivity, the propensity to aggregate and the biological activity of enzymes?
Journal of Molecular Liquids, ISSN: 0167-7322, Vol: 382, Page: 121926
2023
- 7Captures
Metric Options: Counts1 Year3 YearSelecting the 1-year or 3-year option will change the metrics count to percentiles, illustrating how an article or review compares to other articles or reviews within the selected time period in the same journal. Selecting the 1-year option compares the metrics against other articles/reviews that were also published in the same calendar year. Selecting the 3-year option compares the metrics against other articles/reviews that were also published in the same calendar year plus the two years prior.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Captures7
- Readers7
Article Description
The current theories do not provide a convincing explanation for many of the protein' manifestations in solution. Therefore, to shed some light on this critical question, the present work analyzed the effect of dilution on the ability of proteins to catalyze chemical reactions, the so-called biological activity. As models enzymes, laccase from Trametes versicolor and chicken egg white lysozyme were used. The results show that the enzymes' specific biological activity increases on dilution. Three explanations for the intriguing observation were advanced and submitted to experimental scrutiny. Amongst the three hypotheses, only one was corroborated by experiments. According to this explanation, when dissolved in water, proteins reveal two populations: one biologically active whose relative occurrence increases on dilution and another which is not active and whose molecular proportion varies in the opposite direction. Therefore, the reported experimental facts strongly support the chemical behaviour of the proteins in the solution. According to the herein-advocated concepts, they could undergo a dissociation process similar to that found in electrolyte chemistry.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0167732223007298; http://dx.doi.org/10.1016/j.molliq.2023.121926; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85157983865&origin=inward; https://linkinghub.elsevier.com/retrieve/pii/S0167732223007298; https://dx.doi.org/10.1016/j.molliq.2023.121926
Elsevier BV
Provide Feedback
Have ideas for a new metric? Would you like to see something else here?Let us know