Migration mechanism of microcystin-LR synergism with Cu 2+ from algal cell to water environment using modified nano system

Effects on various macromolecules in algal cells occurred when heavy metals migrated to algal cells from the water environment. An innovative nanosystem was introduced to explore the synergistic process of MC-LR and Cu 2+ in algal cells. In this method, the complex groups of Cu 2+ and MC-LR were deeply analyzed by infrared spectroscopy, and the migration process and mechanism of MC-LR in cells were explored. The complexation of MC-LR with Cu 2+ changed the exposure of hydrophobic and hydrophilic residues and affected their migration from intracellular to aqueous environment. Cu 2+ mainly bound to =N-H in guanidine group of MC-LR, which changed the exposure of hydrophobic and hydrophilic residues of MC-LR and affected its migration from intracellular to water environment. In this paper, the effects of Cu 2+ concentration and pH on the optimal coordination of MC-LR were studied. When the concentration of Cu 2+ was 0.05 mm-2 mm, the complex of Cu 2+ and MC-LR had synergistic amplification effect on the detection signal of the nanosystem, and the relative signal value reached the maximum when the pH value was 7. Part of the free copper ions complexed with MC-LR with changing conformation before migrating to the water environment.