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Application of digital holographic microscopy to evaluate the dynamics of a single red blood cell influenced by low-power laser light

Optics & Laser Technology, ISSN: 0030-3992, Vol: 142, Page: 107262
2021
  • 6
    Citations
  • 0
    Usage
  • 19
    Captures
  • 0
    Mentions
  • 1
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    6
    • Citation Indexes
      6
  • Captures
    19
  • Social Media
    1
    • Shares, Likes & Comments
      1
      • Facebook
        1

Article Description

In the paper, a shift of individual human erythrocytes drifts speed, stimulated by various low-power lasers irradiation, was studied by digital holographic microscopy (DHM). Well known in physiotherapy, a low-level-laser-therapy (LLLT) scanner was used as the stimulation light source, with diode and He-Ne lasers to compare. In the article, an open-source image processing algorithm for simultaneous dynamics and geometrical properties evaluations of the red blood cells is proposed. A strong influence of the low-level red light on the red blood cells dynamics was observed. The effect is important for a better understanding of the laser treatment mechanism. The proposed phase image analysis algorithm significantly improves the individual cells tracing precision for a life science application. The programme might be useful for optical tweezer, imaging flow cytometry, or high precision cells-photon interaction research.

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