The combined effects of copper and zinc on Arabidopsis involve differential regulation of chlorophyll synthesis and photosystem function

Copper (Cu) and zinc (Zn) are both oxidation-reducing metal elements that are necessary for plant growth, and their effect often depends on their concentration. However, there are few studies that have investigated how plants are stressed and affected when the two ions are present simultaneously, especially when one ion is beneficial due to a low concentration and the other is detrimental due to a high concentration. To address this question, we treated Arabidopsis plants with either high or/and low concentrations of the two ions and investigated their mutual effects and the underlying molecular mechanism, focusing on photosynthetic function. The results showed that the photosynthetic pigment content and the performance of photosynthetic systems were most affected when both metal ions were present at detrimental concentrations (60 μM Cu for Cu 60 and 350 μM Zn for Zn 350 ). These include the effective openness of the photoreaction center, the electron transport rate and efficiency of photosystem II (PSII), the NPQ-dependent energy dissipation and the activity of photosystem I (PSI). However, when the harmful concentration of one of the two metals is combined with the beneficial concentration of the other metal (Cu 5 +Zn 350 or Zn 50 +Cu 60 ), these photosynthetic indicators are compensated to different degrees but the negative effects of copper ions at high dose are more difficult to eliminate than zinc ions. These results were also confirmed by gene expression analysis, which provides a clue to understanding the interaction between heavy metal ions, reducing metal toxicity and improving the tolerance of plants to heavy metals in practice.