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Electrochromatographic separation of hydrophobic amino acid enantiomers by molecularly imprinted capillary columns

Process Biochemistry, ISSN: 1359-5113, Vol: 92, Page: 69-77
2020
  • 12
    Citations
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  • 17
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Metrics Details

  • Citations
    12
    • Citation Indexes
      12
  • Captures
    17

Article Description

In the present study, the enantiomeric forms of hydrophobic amino acids ( l -tryptophan, l -tyrosine, and l -phenylalanine) were separated by molecularly imprinted capillary columns (MICC) via Capillary Electrochromatography (CEC) for the first time. The monomer ratio, crosslinker ratio, template molecule ratio, the porogen ratio and type, polymerization time, and also the effect of temperature were examined to increase the permeability properties of MICC. FTIR, SEM and BET analyses were realized for the characterization of MICC. The effect of the electric field, organic solvent ratio, and pressure were carried out experimentally to determine the optimum conditions. The separation performances of MICC and the non-imprinted capillary columns (NICC) were compared electrochromatographically.

Bibliographic Details

Koray Şarkaya; Süleyman Aşir; Ilgım Göktürk; Fatma Yilmaz; Handan Yavuz; Adil Denizli

Elsevier BV

Chemical Engineering; Biochemistry, Genetics and Molecular Biology; Immunology and Microbiology

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