Investigation of improved uricase release and kinetic parameters through dual affected responsive nanopolymers
Process Biochemistry, ISSN: 1359-5113, Vol: 131, Page: 52-58
2023
- 4Citations
- 3Captures
Metric Options: CountsSelecting the 1-year or 3-year option will change the metrics count to percentiles, illustrating how an article or review compares to other articles or reviews within the selected time period in the same journal. Selecting the 1-year option compares the metrics against other articles/reviews that were also published in the same calendar year. Selecting the 3-year option compares the metrics against other articles/reviews that were also published in the same calendar year plus the two years prior.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Article Description
Uricase suppresses the harmful effects of uric acid accumulation by the degradation of uric acid to allantoin. Maintaining enzyme stability is a major challenge. The stability issue can be avoided when using enzymes loaded into polymeric structures. For this purpose, uricase loaded into responsive nanopolymers (UO-RNPs) was synthesized. The characterizations such as SEM, FTIR, and zeta potential of UO-RNPs were performed. Maximum uricase loading to RNPs was investigated and the release performance of UO-RNPs also under variable conditions such as pH and temperature. On the other hand, loading yield (LY) and loading efficiency (LE) was founded 73.22 ± 3.62% and 71.24 ± 4.61%, while storage stability and reusability of the UO-RNPs were found to be about 68% and 51% of the original activity after 4 weeks and 10 cycles, respectively. From Lineweaver-Burk plot the Km was founded 0.192 and 0.327 mM for loaded and free uricase. While V max was founded 0.131 and 0.0714 μM/min for free uricase and UO-RNPs, respectively. UO-RNPs showed promising matrices for high catalytic efficiency and enhanced stability. Up until now, as far as we know, the present study of the loaded uricase into responsive nanopolymers is the first attempt of uricase enzyme in such an investigation.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S1359511323001939; http://dx.doi.org/10.1016/j.procbio.2023.06.003; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85161680967&origin=inward; https://linkinghub.elsevier.com/retrieve/pii/S1359511323001939; https://dx.doi.org/10.1016/j.procbio.2023.06.003
Elsevier BV
Provide Feedback
Have ideas for a new metric? Would you like to see something else here?Let us know