The neonicotinoid insecticide imidacloprid, but not salinity, impacts the immune system of Sydney rock oyster, Saccostrea glomerata

The broad utilisation of neonicotinoids, particularly imidacloprid (IMI), in agriculture has led to unplanned contamination of aquatic systems around the world. The sublethal effects of individual pesticides on the immune system of oysters, as well as their combined effects with other environmental stressors that fluctuate in estuarine environments, such as salinity, are yet to be investigated in ecotoxicology. We investigated the acute (4 d) toxicity of IMI in two salinity regimes on the immune parameters of Sydney rock oysters (SRO), including total hemocyte counts (THC), differential hemocyte counts (DHC), phagocytosis and hemocyte aggregation (HA), hemolymph protein expression and enzyme (catalase (CAT), glutathione S-transferase (GST) and acetylcholinesterase (AChE)) activities. Environmentally relevant concentrations of IMI were found to cause an increase in THC, induce GST activity, reduce HA, and inhibit AChE activity. However, DHC, CAT activity and phagocytosis were not significantly impacted at any test concentration at either salinity. IMI concentrations ≥0.01 mg/L significantly altered the expression of 28 proteins in the hemolymph of SRO, including an increase in the relative expression of extracellular superoxide dismutase, severin, ATP synthase subunit beta, as well as stress response proteins (heat shock proteins, serine/threonine-protein kinase DCLK3 and peroxiredoxin-1), and a decrease/absence of collagen alpha-4 (VI) and alpha-6 (VI) chain, metalloendopeptidase, L-ascorbate oxidase, transporter, CEP209_CC5 domain-containing protein and actin. This study indicates that the immune system of SRO can be impacted at environmentally relevant concentrations of IMI, but reduced salinity does not appear to influence the toxicity of this insecticide.