Investigating antibiotics, antibiotic resistance genes in soil, groundwater and vegetables in relation to agricultural field - Applicated with lincomycin mycelial residues compost

Antibiotic mycelial residue, a kind of organic bio-waste, after composting with the subsequent land application is an effective way to achieve its resource utilization. However, its influences on soil quality and ecological safety in the practical agricultural field and related environmental media, e.g., groundwater and vegetables, remain investigated. In the present study, a field experiment with vegetable plants was conducted to study the influences of lincomycin mycelial residue compost (LMRC) on soil quality, and antibiotics and ARGs' fate. In particular, soil physicochemical properties and microbial community composition were analyzed. Moreover, antibiotics and ARGs' evolution in soil, vegetable, and groundwater were determined. The results showed that the LMRC amendment enhanced soil fertility with the increases of organic matter, total nitrogen, and available P/K. Enzyme activities except catalase and urease were promoted, and they were positively related to the LMRC application ratio. Soil microbial community composition presented temporary shifts as LMRC added, and the low application amount soil showed no significant difference with control at the end of the experiment. Similarly, lincomycin concentration in soil was far lower than the background, and it decreased below the predicted no-effect concentration in groundwater. Besides, the detected lincomycin in pakchoi grew in 0.5% and 1% LMRC amended soil was lower than acceptable daily intake (30 μg/kg). Low application rate (0.5%) of LMRC caused no significant changes of tested ARGs in soil, vegetables, and groundwater. Information obtained from this study provides reasonable application strategies for LMRC that with environmental acceptable antibiotic and ARGs.