Co-composting of faecal sludge and carbon-rich wastes in the earthworm's synergistic cooperation system: Performance, global warming potential and key microbiome

Composting is an effective alternative for recycling faecal sludge into organic fertilisers. A microflora–earthworm ( Eisenia fetida ) synergistic cooperation system was constructed to enhance the composting efficiency of faecal sludge. The impact of earthworms and carbon-rich wastes (rice straw (RS) and sawdust (S)) on compost properties, greenhouse gas emissions, and key microbial species of composting were evaluated. The addition of RS or S promoted earthworm growth and reproduction. The earthworm-based system reduced the volatile solid of the final substrate by 13.19–16.24 % and faecal Escherichia coli concentrations by 1.89–3.66 log10 cfu/g dry mass compared with the earthworm-free system. The earthworm-based system increased electrical conductivity by 0.322–1.402 mS/cm and reduced C/N by 56.16–64.73 %. The NH 4 + :NO 3 − ratio of the final faecal sludge and carbon-rich waste was <0.16. The seed germination index was higher than 80 %. These results indicate that earthworms contribute to faecal sludge maturation. Earthworm addition reduced CO 2 production. The simultaneous addition of earthworms and RS system (FRS2) resulted in the lowest global warming potential (GWP). The microbial diversity increased significantly over time in the RS-only system, whereas it initially increased and later decreased in the FRS2 system. Cluster analysis revealed that earthworms had a more significant impact on the microbial community than the addition of carbon-rich waste. Co-occurrence networks for earthworm-based systems were simple than those for earthworm-free systems, but the major bacterial genera were more complicated. Highly abundant key species ( norank_f_Chitinophagaceae and norank_f_Gemmatimonadaceae ) are closely related. Microbes may be more cooperative than competitive, facilitating the conversion of carbon and nitrogen in earthworm-based systems. This work has demonstrated that using earthworms is an effective approach for promoting the efficiency of faecal sludge composting and reducing GWP.