Visualized and precise design of artificial small RNAs for regulating T7 RNA polymerase and enhancing recombinant protein folding in Escherichia coli
Synthetic and Systems Biotechnology, ISSN: 2405-805X, Vol: 1, Issue: 4, Page: 265-270
2016
- 2Citations
- 21Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations2
- Citation Indexes2
- CrossRef1
- Captures21
- Readers21
- 21
Article Description
Small non-coding RNAs (sRNAs) have received much attention in recent years due to their unique biological properties, which can efficiently and specifically tune target gene expressions in bacteria. Inspired by natural sRNAs, recent works have proposed the use of artificial sRNAs (asRNAs) as genetic tools to regulate desired gene that has been applied in several fields, such as metabolic engineering and bacterial physiology studies. However, the rational design of asRNAs is still a challenge. In this study, we proposed structure and length as two criteria to implement rational visualized and precise design of asRNAs. T7 expression system was one of the most useful recombinant protein expression systems. However, it was deeply limited by the formation of inclusion body. To settle this problem, we designed a series of asRNAs to inhibit the T7 RNA polymerase (Gene1) expression to balance the rate between transcription and folding of recombinant protein. Based on the heterologous expression of Aspergillus oryzae Li-3 glucuronidase in E. coli, the asRNA-antigene1-17bp can effectively decrease the inclusion body and increase the enzyme activity by 169.9%.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S2405805X1630028X; http://dx.doi.org/10.1016/j.synbio.2016.08.005; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85046238053&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/29062952; https://linkinghub.elsevier.com/retrieve/pii/S2405805X1630028X; https://dx.doi.org/10.1016/j.synbio.2016.08.005
Elsevier BV
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