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An improved process for the release of synthetic DNA sequences from a solid-phase capture support

Tetrahedron Letters, ISSN: 0040-4039, Vol: 106, Page: 154077
2022
  • 4
    Citations
  • 0
    Usage
  • 4
    Captures
  • 1
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    4
    • Citation Indexes
      4
  • Captures
    4
  • Mentions
    1
    • News Mentions
      1
      • 1

Most Recent News

Findings from Food and Drug Administration Yields New Data on Life Science (An Improved Process for the Release of Synthetic Dna Sequences From a Solid-phase Capture Support)

2022 OCT 31 (NewsRx) -- By a News Reporter-Staff News Editor at CDC & FDA Daily -- A new study on Life Science is now

Article Description

To facilitate the solid-phase purification of synthetic DNA sequences, a riboside phosphoramidite, carrying a 5- O -capture linker and a 2- O -silyl ether protecting group, is incorporated into a DNA sequence during its last solid-phase synthesis cycle. After deprotection and release of the DNA sequence from the synthesis support, the sequence is then covalently linked to a capture support to enable the removal of shorter unbound DNA sequences by simply washing these off the support. The solid-phase purified DNA sequence is then released from the capture support, through an innovative intramolecular cyclodeesterification of its terminal riboside ethyl phosphate triester entity and is isolated in a yield of 94% while displaying an exquisite purity of 97%.

Bibliographic Details

Andrzej Grajkowski; Brian M. Cawrse; Mayumi Takahashi; Serge L. Beaucage

Elsevier BV

Biochemistry, Genetics and Molecular Biology; Pharmacology, Toxicology and Pharmaceutics; Chemistry

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