An improved process for the release of synthetic DNA sequences from a solid-phase capture support
Tetrahedron Letters, ISSN: 0040-4039, Vol: 106, Page: 154077
2022
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Most Recent News
Findings from Food and Drug Administration Yields New Data on Life Science (An Improved Process for the Release of Synthetic Dna Sequences From a Solid-phase Capture Support)
2022 OCT 31 (NewsRx) -- By a News Reporter-Staff News Editor at CDC & FDA Daily -- A new study on Life Science is now
Article Description
To facilitate the solid-phase purification of synthetic DNA sequences, a riboside phosphoramidite, carrying a 5- O -capture linker and a 2- O -silyl ether protecting group, is incorporated into a DNA sequence during its last solid-phase synthesis cycle. After deprotection and release of the DNA sequence from the synthesis support, the sequence is then covalently linked to a capture support to enable the removal of shorter unbound DNA sequences by simply washing these off the support. The solid-phase purified DNA sequence is then released from the capture support, through an innovative intramolecular cyclodeesterification of its terminal riboside ethyl phosphate triester entity and is isolated in a yield of 94% while displaying an exquisite purity of 97%.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0040403922005317; http://dx.doi.org/10.1016/j.tetlet.2022.154077; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85136136926&origin=inward; https://linkinghub.elsevier.com/retrieve/pii/S0040403922005317; https://dx.doi.org/10.1016/j.tetlet.2022.154077
Elsevier BV
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