Modulation of equine articular chondrocyte messenger RNA levels following brief exposures to recombinant equine interleukin-1β
Veterinary Immunology and Immunopathology, ISSN: 0165-2427, Vol: 106, Issue: 1, Page: 23-38
2005
- 6Citations
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Article Description
The effect of recombinant equine IL-1β (EqIL-1β) on steady-state mRNA levels of equine articular chondrocytes in high-density monolayer culture was investigated using a customized cDNA array analysis. Total RNA samples isolated from chondrocytes cultured in media alone or with the addition of 1 ng/ml EqIL-1β for 1-, 3-, and 6-h durations of exposure were reverse transcribed, radiolabeled, and hybridized to a customized 380-target cDNA array. Means of duplicate log base 2 transformed hybridization signals were normalized to equine glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mean signal intensities. Differentially expressed transcripts were identified using a two-stage mixed linear analysis of variance model (Statistical Analysis Software, Cary, NC). A time-dependent pattern was observed in the number of transcripts increased ≥two-fold in response to EqIL-1β after 1, 3 and 6 h (1, 2 and 109 transcripts, respectively). At 6 h of EqIL-1β stimulation, signal intensities for 88 cDNA targets with purported function in processes related to cell cycle, intracellular signaling, transcription, translation, extracellular matrix turnover, and inflammation, as well as a number of cDNAs lacking homology to previously reported cDNA sequences, were increased >two-fold and were associated with p < 0.05. Principal component analysis identified a vector component (∼10% of the total variation) corresponding to a potential EqIL-1β co-regulation of cell cycle associated gene transcription. These results support and expand our existing comprehension of the complex role of IL-1 in modulated chondrocyte gene expression and suggest the involvement of specific target gene up-regulation and activation of downstream inflammatory cascade mediators. This study adds to the current understanding of the molecular events associated with an IL-1 induced inflammation and pathobiologic processes that may be associated with the development of equine osteoarthritis.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0165242705000243; http://dx.doi.org/10.1016/j.vetimm.2005.01.003; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=19344366245&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/15910990; https://linkinghub.elsevier.com/retrieve/pii/S0165242705000243; https://dx.doi.org/10.1016/j.vetimm.2005.01.003
Elsevier BV
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