Development of a genetic system for the archaeal virus Sulfolobus turreted icosahedral virus (STIV)
Virology, ISSN: 0042-6822, Vol: 415, Issue: 1, Page: 6-11
2011
- 26Citations
- 35Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations26
- Citation Indexes26
- 26
- CrossRef23
- Captures35
- Readers35
- 35
Article Description
Our understanding of archaeal viruses has been limited by the lack of genetic systems for examining viral function. We describe the construction of an infectious clone for the archaeal virus Sulfolobus turreted icosahedral virus (STIV). STIV was isolated from a high temperature (82 °C) acidic (pH 2.2) hot spring in Yellowstone National Park and replicates in the archaeal model organism Sulfolobus solfataricus ( Rice et al., 2004 ). While STIV is one of most studied archaeal viruses, little is known about its replication cycle. The development of an STIV infectious clone allows for directed gene disruptions and detailed genetic analysis of the virus. The utility of the STIV infectious clone was demonstrated by gene disruption of STIV open reading frame (ORF) B116 which resulted in crippled virus replication, while disruption of ORFs A197, C381 and B345 was lethal for virus replication.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0042682211001486; http://dx.doi.org/10.1016/j.virol.2011.03.023; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=79956189450&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/21496857; https://linkinghub.elsevier.com/retrieve/pii/S0042682211001486; https://dx.doi.org/10.1016/j.virol.2011.03.023
Elsevier BV
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