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Protocol for global proteome, virome, and metaproteome profiling of respiratory specimen (VTM) in COVID-19 patient by LC-MS/MS-based analysis

STAR Protocols, ISSN: 2666-1667, Vol: 3, Issue: 1, Page: 101045
2022
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Article Description

In this protocol, we describe global proteome profiling for the respiratory specimen of COVID-19 patients, patients suspected with COVID-19, and H1N1 patients. In this protocol, details for identifying host, viral, or bacterial proteome (Meta-proteome) are provided. Major steps of the protocol include virus inactivation, protein quantification and digestion, desalting of peptides, high-resolution mass spectrometry (HRMS)-based analysis, and downstream bioinformatics analysis. For complete details on the use and execution of this profile, please refer to Maras et al. (2021).

Bibliographic Details

Tripathi, Gaurav; Sharma, Nupur; Bindal, Vasundhra; Yadav, Manisha; Mathew, Babu; Sharma, Shvetank; Gupta, Ekta; Singh Maras, Jaswinder; Sarin, Shiv Kumar

Elsevier BV

Neuroscience; Biochemistry, Genetics and Molecular Biology; Immunology and Microbiology

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