Polar metabolomics in human muscle biopsies using a liquid-liquid extraction and full-scan LC-MS
STAR Protocols, ISSN: 2666-1667, Vol: 3, Issue: 2, Page: 101302
2022
- 19Citations
- 27Captures
- 2Mentions
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations19
- Citation Indexes19
- CrossRef19
- 18
- Captures27
- Readers27
- 27
- Mentions2
- References2
- Wikipedia2
Article Description
We describe here a user-friendly analysis protocol for semi-targeted polar metabolomics in human muscle biopsies using Zwitterionic Hydrophilic Interaction Liquid Chromatography and high-resolution full-scan mass spectrometry. Previously, this protocol has been used for Caenorhabditis elegans. Here we show that it can be successfully applied to human muscle biopsies with minor adjustments. Summarized instructions for other matrices are also provided. As peak integration in metabolomics can be challenging, we provide expected retention times and extensive peak descriptions to aid this process. For complete details on the use and execution of this protocol, please refer to Molenaars et al. (2021).
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S2666166722001824; http://dx.doi.org/10.1016/j.xpro.2022.101302; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85128412776&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/35479116; https://linkinghub.elsevier.com/retrieve/pii/S2666166722001824; https://dx.doi.org/10.1016/j.xpro.2022.101302
Elsevier BV
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