Protocol for live-cell imaging of immune synapse formation and activation of CAR T cells against cancer cells
STAR Protocols, ISSN: 2666-1667, Vol: 5, Issue: 4, Page: 103422
2024
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
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Article Description
Immune synapse (IS) formation determines T cell antitumor activity. Here, we present a protocol for characterizing the IS formation between chimeric antigen receptor (CAR) T cells and tumor cells by measuring the IS size and calcium flux by live-cell imaging. We describe steps for CAR T cell manufacturing, sample preparation, image acquisition, and data analysis. For complete details on the use and execution of this protocol, please refer to Chockley et al., 1 Ibanez et al., 2 and Zoine et al. 3
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S2666166724005872; http://dx.doi.org/10.1016/j.xpro.2024.103422; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85208032472&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/39488837; https://linkinghub.elsevier.com/retrieve/pii/S2666166724005872; https://dx.doi.org/10.1016/j.xpro.2024.103422
Elsevier BV
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