Complete, high-quality genomes from long-read metagenomic sequencing of two wolf lichen thalli reveals enigmatic genome architecture
Genomics, ISSN: 0888-7543, Vol: 112, Issue: 5, Page: 3150-3156
2020
- 16Citations
- 58Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations16
- Citation Indexes16
- 16
- CrossRef7
- Captures58
- Readers58
- 58
Article Description
Fungal genomes display incredible levels of complexity and diversity, and are exceptional study systems for genome evolution. Here we used the Oxford Nanopore MinION sequencing platform to generate high-quality fungal genomes from complex metagenomic samples of lichen thalli. We sequenced two wolf lichens using one flow cell per sample, generating 17.1 Gbps for Letharia lupina and 14.3 Gbps for Letharia columbiana. The resulting L. lupina genome is one of the most contiguous lichen genomes available to date, with 49.2 Mbp contained on 31 contigs. The L. columbiana genome, while less contiguous, is still relatively high quality, with 52.3 Mbp on a total of 161 contigs. Each thallus for both species contained multiple distinct haplotypes, a phenomenon that has rarely been empirically demonstrated. The Oxford Nanopore sequencing technologies are robust and effective when applied to complex symbioses, and have the potential to fundamentally transform our understanding of fungal genetics.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0888754320304614; http://dx.doi.org/10.1016/j.ygeno.2020.06.006; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85086123117&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/32504651; https://linkinghub.elsevier.com/retrieve/pii/S0888754320304614; https://dx.doi.org/10.1016/j.ygeno.2020.06.006
Elsevier BV
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