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Optogenetic reprogramming of carbon metabolism using light-powering microbial proton pump systems

Metabolic Engineering, ISSN: 1096-7176, Vol: 72, Page: 227-236
2022
  • 15
    Citations
  • 0
    Usage
  • 25
    Captures
  • 4
    Mentions
  • 3
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    15
  • Captures
    25
  • Mentions
    4
    • News Mentions
      3
      • News
        3
    • Blog Mentions
      1
      • Blog
        1
  • Social Media
    3
    • Shares, Likes & Comments
      3
      • Facebook
        3

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Article Description

In microbial fermentative production, ATP regeneration, while crucial for cellular processes, conflicts with efficient target chemical production because ATP regeneration exhausts essential carbon sources also required for target chemical biosynthesis. To wrestle with this dilemma, we harnessed the power of microbial rhodopsins with light-driven proton pumping activity to supplement with ATP, thereby facilitating the bioproduction of various chemicals. We first demonstrated a photo-driven ATP supply and redistribution of metabolic carbon flows to target chemical synthesis by installing already-known delta rhodopsin (dR) in Escherichia coli. In addition, we identified novel rhodopsins with higher proton pumping activities than dR, and created an engineered cell for in vivo self-supply of the rhodopsin-activator, all- trans -retinal. Our concept exploiting the light-powering ATP supplier offers a potential increase in carbon use efficiency for microbial productions through metabolic reprogramming.

Bibliographic Details

Toya, Yoshihiro; Hirono-Hara, Yoko; Hirayama, Hidenobu; Kamata, Kentaro; Tanaka, Ryo; Sano, Mikoto; Kitamura, Sayaka; Otsuka, Kensuke; Abe-Yoshizumi, Rei; Tsunoda, Satoshi P; Kikukawa, Hiroshi; Kandori, Hideki; Shimizu, Hiroshi; Matsuda, Fumio; Ishii, Jun; Hara, Kiyotaka Y

Elsevier BV

Biochemistry, Genetics and Molecular Biology; Chemical Engineering; Immunology and Microbiology

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