Enzymatic reactions in liposomes using the detergent-induced liposome loading method
Biochimica et Biophysica Acta (BBA) - Biomembranes, ISSN: 0005-2736, Vol: 1416, Issue: 1, Page: 57-68
1999
- 41Citations
- 29Captures
Metric Options: Counts1 Year3 YearSelecting the 1-year or 3-year option will change the metrics count to percentiles, illustrating how an article or review compares to other articles or reviews within the selected time period in the same journal. Selecting the 1-year option compares the metrics against other articles/reviews that were also published in the same calendar year. Selecting the 3-year option compares the metrics against other articles/reviews that were also published in the same calendar year plus the two years prior.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations41
- Citation Indexes41
- 41
- CrossRef28
- Captures29
- Readers29
- 29
Article Description
Microcompartmentalization is a crucial step in the origin of life. More than 30 years ago, Oparin et al. proposed models based on biochemical reactions taking place in so-called coacervates. Their intention was to develop systems with which semipermeable microcompartments could be established. In the present work we follow their intuition, but we use well-characterized bilayer structures instead of the poorly characterized coacervates. Liposomes from phospholipids can be used as microreactors but they exhibit only a modest permeability and, therefore, chemical reactions occurring inside these structures are depleted after a relatively short period. Here it is shown that even highly stable liposomes from 1-palmitoyl-2-oleoyl- sn -glycero-3-phosphocholine (POPC) can be used as semipermeable microreactors when treated with sodium cholate. Using this kind of mixed liposomes, we describe a biochemical reaction occurring inside the liposomes while the same reaction is prevented in the external medium. In addition, we show that this cholate-induced permeability of POPC bilayers can even be used to load macromolecules such as enzymes from the outside.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0005273698002107; http://dx.doi.org/10.1016/s0005-2736(98)00210-7; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0032898073&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/9889319; http://linkinghub.elsevier.com/retrieve/pii/S0005273698002107; http://api.elsevier.com/content/article/PII:S0005273698002107?httpAccept=text/xml; http://api.elsevier.com/content/article/PII:S0005273698002107?httpAccept=text/plain; https://linkinghub.elsevier.com/retrieve/pii/S0005273698002107; http://dx.doi.org/10.1016/s0005-2736%2898%2900210-7; https://dx.doi.org/10.1016/s0005-2736%2898%2900210-7
Elsevier BV
Provide Feedback
Have ideas for a new metric? Would you like to see something else here?Let us know