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Platelet-activating factor stimulation of p125 FAK and p130 Cas tyrosine phosphorylation in brain

Brain Research, ISSN: 0006-8993, Vol: 835, Issue: 2, Page: 275-281
1999
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Article Description

The effect of platelet-activating factor (PAF) on protein tyrosine phosphorylation was studied in rat brain slices. PAF induced a time- and concentration-dependent increase in tyrosine phosphorylation of a doublet of approximately 125 kDa. These proteins were identified by immunoprecipitation as p125 FAK and p130 Cas, using monoclonal antibodies. This effect was mediated by PAF receptors, as shown by its inhibition by the action of a PAF antagonist. The tyrosine phosphorylation evoked by PAF was dependent, at least in part, on external calcium. The involvement of protein kinase C was demonstrated by the synergistic effect of TPA on PAF-stimulated tyrosine phosphorylation. The finding that PAF stimulates tyrosine phosphorylation of both focal adhesion protein p125 FAK and p130 Cas suggests that PAF might modulate the integrin mediated signal transduction in the brain.

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