In Vitro Secretion of Gastrin, Insulin, and Glucagon in Tissue Cultures of Pancreas from a Child With Neonatal Intractable Hypoglycemia
Gastroenterology, ISSN: 0016-5085, Vol: 71, Issue: 2, Page: 255-262
1976
- 15Citations
- 3Captures
Metric Options: CountsSelecting the 1-year or 3-year option will change the metrics count to percentiles, illustrating how an article or review compares to other articles or reviews within the selected time period in the same journal. Selecting the 1-year option compares the metrics against other articles/reviews that were also published in the same calendar year. Selecting the 3-year option compares the metrics against other articles/reviews that were also published in the same calendar year plus the two years prior.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Article Description
Small pieces of pancreatic tissue were obtained at surgery from a subtotal pancreatectomy performed in a 45-day-old child suffering from intractable neonatal hyperinsulinic hypoglycemia. Histological examination was performed using aldehyde fuchsin, Grimelius', and Hellerström-Hellman's stainings, and immunoperoxidase labeling of insulin and gastrin. The pancreatic tissue before explantation showed numerous and sometimes hyperplastic islets, together with isolated insulin-, glucagon-, and gastrin-containing cells scattered among the exocrine tissue, in aspects similar to “B cell nesidioblastosis.” These features could be interpreted as an acinoinsular transformation and/or an embryonic malformation. Extralobular endocrine islet formation by budding from ductular structures was evoked, suggesting the persistency of embryonic properties. The pieces were cultivated on rooster plasma coagulum covered with culture medium. In vitro, endocrine cells survived for 43 days, with outgrowth from the explant and with retention of their secretory abilities. After each medium renewal, radioimmunoassays were performed on the culture medium; they showed that insulin and glucagon secretions decreased with time. On the contrary, secretion of immunoreactive gastrin progressively increased, and kept up to 43 days, with subcultures. Some explants developed in a peculiar way, outgrowing as epithelial layers rich in gastrin-secreting cells as indicated by radioimmunoassays performed after they were reexplanted.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0016508576801989; http://dx.doi.org/10.1016/s0016-5085(76)80198-9; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0017155021&origin=inward; https://linkinghub.elsevier.com/retrieve/pii/S0016508576801989; https://api.elsevier.com/content/article/PII:S0016508576801989?httpAccept=text/xml; https://api.elsevier.com/content/article/PII:S0016508576801989?httpAccept=text/plain; https://dul.usage.elsevier.com/doi/; http://dx.doi.org/10.1016/s0016-5085%2876%2980198-9; https://dx.doi.org/10.1016/s0016-5085%2876%2980198-9
Elsevier BV
Provide Feedback
Have ideas for a new metric? Would you like to see something else here?Let us know