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Metabolic Functions of Myo -inositol

Journal of Biological Chemistry, ISSN: 0021-9258, Vol: 246, Issue: 2, Page: 461-465
1971
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  • Citations
    8
    • Citation Indexes
      8
      • CrossRef
        8

Article Description

The roles of K + and the Na + - and K + -activated adenosine triphosphatase (Na + -K + pump) in the transport of α-aminoisobutyric acid (AIB) in KB cells were evaluated. It was shown that 0.33 µ m ouabain causes half-maximal inhibition of the Na + -K + pump. Similar sensitivity to the inhibitor was shown earlier for AIB transport. However, whereas the pump is inhibited maximally within 90 sec, inhibition of AIB transport is time-dependent and is maximal after 30 min of the cells' exposure to the inhibitor. Selective inactivation of the Na + -K + pump by ouabain or by omission of extracellular K + is without effect on the rate of AIB influx, provided that the concentration of intracellular K + remains above 30 m m. Depletion of intracellular K + leads to a loss of concentrative AIB transport. This inactivation is not immediate; rather, it is time-dependent. Subsequent addition of KCl fails to reverse this inactivation, even though the cells can concentrate K + 11-fold to a level greater than 55 m m. The intracellular K + concentration which protects against inactivation of the AIB transport system is above 30 m m. Extracellular K +, other than being the source of intracellular K +, is not required for active AIB transport. It is concluded that the role of the Na + -K + pump in AIB transport is to maintain the necessary concentration of intracellular K + for maintaining the functional integrity of the AIB transport system.

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