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Structural comparison between the trout and mammalian hydrophilic domain of nadphcytochrome P-450 reductase

Journal of Chromatography A, ISSN: 0021-9673, Vol: 397, Issue: C, Page: 123-136
1987
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Article Description

The isolation of the protease-solubilized NADPHcytochrome P-450 reductase from trout liver and its properties are described. The sequence of the “hydrophilic domain” [protease-solubilized NADPHcytochrome P-450 reductase from trout (residues Lys 56 —Ser 678 )] is reported. The CNBr fragments of the trout “hydrophilic domain” and their proteolytic subpeptides were sequenced. The CNBr fragments were aligned by homology to the reported sequence of the porcine NADPHcytochrome P-450 reductase. The structures of the mammalian and the trout NADPHcytochrome P-450 reductases were compared. Stretches with high exchange rates between the pig and trout reductase were found at the NH 2 and the COOH terminal regions of the hydrophilic domain.

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