PlumX Metrics
Embed PlumX Metrics

Leukotactin-1-induced ERK activation is mediated via G i /G o protein/PLC/PKCδ/Ras cascades in HOS cells

Life Sciences, ISSN: 0024-3205, Vol: 73, Issue: 4, Page: 447-459
2003
  • 21
    Citations
  • 0
    Usage
  • 5
    Captures
  • 0
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    21
    • Citation Indexes
      21
  • Captures
    5

Article Description

Recently cloned leukotactin-1 (Lkn-1) that belongs to CC chemokine family has not been characterized. To understand the intracellular events following Lkn-1 binding to CCR1, we investigated the activities of signaling molecules in response to Lkn-1 in human osteogenic sarcoma cells expressing CCR1. Lkn-1-stimulated cells showed elevated phosphorylation of extracellular signal-related kinases (ERK1/2) with a distinct time course. ERK activation was peaked in 30 min and 12 h showing biphasic activation of ERK. Pertussis toxin, an inhibitor of G i /G o protein, and phospholipase C (PLC) inhibitor blocked Lkn-1-induced activation of ERK. Protein kinase Cδ (PKCδ) specific inhibitor rottlerin inhibited ERK activation in Lkn-1-stimulated cells. The activities of PLC and PKCδ were also enhanced by Lkn-1 stimulation. Dominant negative Ras inhibited activation of ERK. Immediate early response genes such as c-fos and c-myc were induced by Lkn-1 stimulation. Lkn-1 affected the cell cycle progression by cyclin D 3 induction. These results suggest that Lkn-1 activates the ERK pathway by transducing the signal through G i /G o protein, PLC, PKCδ and Ras, and it may play a role for cell proliferation, differentiation, and regulation of gene expression for other cellular processes.

Provide Feedback

Have ideas for a new metric? Would you like to see something else here?Let us know