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Proteolytic specificity of rhodostoxin, the major hemorrhagin of Calloselasma rhodostoma (Malayan pit viper) venom

Toxicon, ISSN: 0041-0101, Vol: 35, Issue: 6, Page: 979-984
1997
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Article Description

The proteolytic specificity of rhodostoxin, the major hemorrhagin from Calloselasma rhodostoma (Malayan pit viper) venom was investigated using oxidized B-chain of bovine insulin as substrate. Six peptide bonds were cleaved: Ser 9 -Hist 10, His 10 -Leu 11, Ala 14 -Leu 15, Tyr 16 -Leu 17, Gly 20 -Glu 21 and Phe 24 -Phe 25. Deglycosylated rhodostoxin, however, cleaved primarily at Arg 22 -Gly 23.

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