PlumX Metrics
Embed PlumX Metrics

Molecular approaches to structure-function analysis of interleukin-8

Methods in Enzymology, ISSN: 0076-6879, Vol: 287, Page: 45-58
1997
  • 10
    Citations
  • 0
    Usage
  • 3
    Captures
  • 0
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

Article Description

By constructing and measuring receptor-binding affinities of IL-8 and melanoma growth stimulating activity variants, this chapter shows that the N-loop region of IL-8 is required for binding to CXCR1. The conformation of the N-loop appears critical for CXCR1 binding, based on the need for Leu-49, which packs against the N-loop residues Tyr-13 and Phe-17. The N-terminal domains of CXCR1 and CXCR2 have been previously shown to interact with IL-8 and to play a role in determining chemokine-binding specificity. The N-loop region of IL-8 therefore, interacts directly with the N-terminal domains of the IL-8 receptors. Based on the available data the chapter speculates that there is a one-to-one interaction of the secondary N-loop site on IL-8 with a portion of the CXCR1 N-terminal domain and that the ELR residues interact with a region of charged residues, Arg-199, Arg-203, and Asp-265, identified by alanine scanning mutagenesis in CXCRI extracellular loops 3 and 4.

Provide Feedback

Have ideas for a new metric? Would you like to see something else here?Let us know