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A radioimmunoassay determination of the concentrations of prostaglandins E 2 and F 2α in painful and asymptomatic human dental pulps

Journal of Endodontics, ISSN: 0099-2399, Vol: 11, Issue: 8, Page: 330-335
1985
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Article Description

The purpose of this study was to determine the concentrations of prostaglandin E 2 and prostaglandin F 2α in painful and asymptomatic human dental pulps. Pulps were obtained from three groups of teeth: uninflamed pulps, asymptomatic teeth with caries and/or large restorations, and symptomatic teeth with the clinical diagnosis of irreversible pulpitis. Pulps were dissected from the teeth and stored in liquid nitrogen. They were homogenized in 40% ethanol and a lipid solvent extraction was performed. Prostaglandin levels were measured using radioimmunoassay. The pulps which were likely to demonstrate inflammation had significantly higher mean concentrations of prostaglandin E 2 than did the uninflamed pulps (p<0.05). Painful pulps had a much higher level of both prostaglandin E 2 and prostaglandin F 2α than the asymptomatic pulps (p < 0.01).

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