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Chapter 2A Nonenzymatic cofactors: factor V * *Supported by HL-17430 and HL-34575.

New Comprehensive Biochemistry, ISSN: 0167-7306, Vol: 13, Issue: C, Page: 15-34
1986
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    5
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      5

Article Description

This chapter highlights factor V. In human blood, factor V is divided between two principal compartments: the blood plasma and the platelet. A small fraction of factor V is also contained within the white cell populations in human blood. The bovine aortic endothelial cells grown in culture synthesize and secrete factor V. In contrast, human umbilical vein endothelium synthesizes but does not secrete factor V. Factor V synthesis has also been demonstrated in human hepatocellular carcinoma cell line (HepG2). The other major site of factor V synthesis appears to be within the bone marrow stem cell pool. Factor V is regarded a large, single-chain molecule, with a molecular weight of 330,000. At least three proteases associated with blood clotting can cleave the factor V peptide chain. Factor V and factor VIII:C appear to be homologous in terms of their function as cofactors in reactions involving vitamin K-dependent enzymes and vitamin K-dependent substrates.

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