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Numerous and highly developed tubular projections from plastids observed in Tobacco epidermal cells

Plant Science, ISSN: 0168-9452, Vol: 160, Issue: 3, Page: 449-454
2001
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Article Description

Tubular projections from plastids (stromules) were observed using a stroma-targeted green fluorescent protein (GFP) fusion protein and a confocal laser scanning microscope. In tobacco ( Nicotiana tabacum ) epidermal cells, stromules were observed at a high frequency. Some of them were long and connected plastids. Three days after particle bombardment, 80.6±6.99% of the transformed cells contained some plastids with more than one stromule, and 40.2±7.7% contained at least one pair of plastids connected by stromules. In a few cells, numerous and highly developed stromules covering the whole cell were observed. Stromules were also observed in epidermal cells in each of three other plant species that were tested: rice, dayflower ( Commelina communis ) and Arabidopsis thaliana. These findings demonstrated that stromules are common structure in plant epidermal cells.

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