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Kinetic and pharmacological analysis of l -[ 35 S]cystine transport into rat brain synaptosomes

Neurochemistry International, ISSN: 0197-0186, Vol: 36, Issue: 6, Page: 513-521
2000
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Article Description

The synaptosomal transport of l -[ 35 S]cystine occurs by three mechanisms that are distinguishable on the basis of their ionic dependence, kinetics of transport and the specificity of inhibitors. They are (a) low affinity sodium-dependent transport ( K m 463±86 μM, V max 185±20 nmol mg protein −1 min −1 ), (b) high affinity sodium-independent transport ( K m 6.90±2.1 μM, V max 0.485±0.060 nmol mg protein −1 min −1 ) and (c) low affinity sodium-independent transport ( K m 327±29 μM, V max 4.18±0.25 nmol mg protein −1 min −1 ).

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