A SECIS binding protein (SBP) is distinct from selenocysteyl-tRNA protecting factor (SePF)
Biochimie, ISSN: 0300-9084, Vol: 81, Issue: 3, Page: 213-218
1999
- 26Citations
- 11Captures
Metric Options: CountsSelecting the 1-year or 3-year option will change the metrics count to percentiles, illustrating how an article or review compares to other articles or reviews within the selected time period in the same journal. Selecting the 1-year option compares the metrics against other articles/reviews that were also published in the same calendar year. Selecting the 3-year option compares the metrics against other articles/reviews that were also published in the same calendar year plus the two years prior.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations26
- Citation Indexes26
- 26
- CrossRef15
- Captures11
- Readers11
- 11
Article Description
In mammals, most of the selenium contained in their body is present as an unusual amino acid, selenocysteine (Sec), whose codon is UGA. Because the UGA codon is normally recognized as a translational stop signal, it is intriguing how cells recognize and distinguish the UGA Sec codon from the UGA stop codon. In eukaryotic selenoprotein mRNAs, it has been proposed that a conserved stem-loop structure designated Sec insertion sequence (SECIS) located in the 3`-untranslated regions is required for recognition of UGA as a Sec codon. Although some proteins (SBPs) have been reported to bind to SECIS, it is not clear how the SECIS element can mediate Sec insertion at UGA. Eukaryotic Sec-tRNA Sec is not recognized by elongation factor EF-1α, but is recognized specifically by a Sec-tRNA Sec protecting factor, SePF, in bovine liver extracts. In this study, we provide evidence that SePF is distinct from SBP by chromatography. Upon UV irradiation, the SECIS RNA was cross-linked to a 47.5 kDa protein, a likely candidate of SBP, that is contained in the complex with a molecular mass of 150 kDa. These results suggest that SBP and SePF play different roles for the Sec incorporation. To our knowledge, this is the first demonstration that SBP is discriminated from the factor which directly recognizes Sec-tRNA Sec, providing a novel clue to the mechanism of selenocysteine decoding in eukaryotes.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0300908499800546; http://dx.doi.org/10.1016/s0300-9084(99)80054-6; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0032724547&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/10385002; https://linkinghub.elsevier.com/retrieve/pii/S0300908499800546; http://linkinghub.elsevier.com/retrieve/pii/S0300908499800546; http://api.elsevier.com/content/article/PII:S0300908499800546?httpAccept=text/xml; http://api.elsevier.com/content/article/PII:S0300908499800546?httpAccept=text/plain; http://dx.doi.org/10.1016/s0300-9084%2899%2980054-6; https://dx.doi.org/10.1016/s0300-9084%2899%2980054-6
Elsevier BV
Provide Feedback
Have ideas for a new metric? Would you like to see something else here?Let us know