Formation of 70S ribosomes: large activation energy is required for the adaptation of exclusively the small ribosomal subunit
Biophysical Chemistry, ISSN: 0301-4622, Vol: 96, Issue: 2, Page: 153-161
2002
- 19Citations
- 20Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations19
- Citation Indexes19
- 19
- CrossRef14
- Captures20
- Readers20
- 20
Article Description
Association of ribosomal subunits is an essential reaction during the initiation phase of protein synthesis. Optimal conditions for 70S formation in vitro were determined to 20 mM Mg 2+ and 30 mM K +. Under these conditions, the association reaction proceeds with first order kinetics, suggesting a conformational change to be the rate-limiting step. 70S formation separates into two sub-reactions, the adaptation of the ribosomal subunits to the association conditions and the association step itself. The activation energy of the process was determined to 78 kJ/mol and revealed to be required exclusively for the adaptation of the small subunit, rather than the large subunit or the association step. The presence of mRNA [poly(U)] together with cognate AcPhe-tRNA, accelerates the association rate significantly, forming a well-defined 70S peak in sucrose gradient profiles. mRNA alone provokes an equivalent acceleration, however, the resulting 70S couple impresses as an ill-defined, broad peak, probably indicating the readiness of the ribosome for tRNA binding, upon which the ribosome flips into a defined state.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0301462202000212; http://dx.doi.org/10.1016/s0301-4622(02)00021-2; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0037007466&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/12034437; https://linkinghub.elsevier.com/retrieve/pii/S0301462202000212; http://linkinghub.elsevier.com/retrieve/pii/S0301462202000212; http://api.elsevier.com/content/article/PII:S0301462202000212?httpAccept=text/xml; http://api.elsevier.com/content/article/PII:S0301462202000212?httpAccept=text/plain; http://dx.doi.org/10.1016/s0301-4622%2802%2900021-2; https://dx.doi.org/10.1016/s0301-4622%2802%2900021-2
Elsevier BV
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