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Cloning and characterization of amphibian cold inducible RNA-binding protein

Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, ISSN: 1096-4959, Vol: 125, Issue: 2, Page: 237-245
2000
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Article Description

Gene expression of cold inducible RNA-binding protein (CIRP) was examined in the frog. In Xenopus laevis, expression of CIRP (XCIRP) was observed in both brain and liver at 24°C. Circadian expression of XCIRP was observed in brain. Expression of XCIRP in brain was induced by cold treatment and gradually decreased to the control level at 24°C, but no significant changes were observed in liver. Employing the sequence of murine CIRP, bullfrog ( Rana catesbeiana ) CIRP gene was cloned. The bullfrog CIRP gene, designated BFCIRP, was 706 bp in length and encoded a putative protein of 164 amino acid residues. The deduced protein contained one consensus sequence of RNA-binding domain (CS-RBD) and a glycine rich domain (GRD). The amino acid sequence of BFCIRP was 78.4% identical to XCIRP. Expression of BFCIRP in brain was stronger in winter than that in summer. These findings suggest that BFCIRP expression in brain may link to hibernation.

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