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Crosslinking of enzymes for improved stability and performance

Current Opinion in Biotechnology, ISSN: 0958-1669, Vol: 10, Issue: 4, Page: 331-335
1999
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Article Description

Stabilization of enzymes without diminishing and diluting their activity is crucial for their use as industrial catalysts. One practically proven approach involves crystallization of the enzyme (size 1–200 μm) followed by subsequent crosslinking. In many cases, the resulting crosslinked enzyme crystals exhibit increased storage stability as well as stability in applications. The technology is complementary to protein engineering methods that aim at boosting the inherent stability of the enzyme.

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