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A small peptide inhibitor of DNA replication defines the site of interaction between the cyclin-dependent kinase inhibitor p21 WAF1 and proliferating cell nuclear antigen

Current Biology, ISSN: 0960-9822, Vol: 5, Issue: 3, Page: 275-282
1995
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Background: p21 WAF1 is a potent inhibitor of the cell-cycle regulatory cyclin-dependent kinases (Cdks). It acts on Cdks in the G1 and S phases of the cell cycle, and also binds to proliferating cell nuclear antigen (PCNA), blocking DNA replication in vitro. Transcription of p21 WAF1 can be induced by the human tumour suppressor protein p53, suggesting that the action of p21 WAF1 may be important in cancer prevention. We have investigated the interaction between p21 WAF1 and PCNA using a genetic two-hybrid screen and with arrays of synthetic peptides derived from the p21 WAF1 protein sequence.

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