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Construction of recombinants Pseudomonas putida BO14 and Escherichia coli QEFCA8 for ferulic acid biotransformation to vanillin

Journal of Bioscience and Bioengineering, ISSN: 1389-1723, Vol: 88, Issue: 1, Page: 103-106
1999
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Article Description

Recombinants Pseudomonas putida BO14 and Escherichia coli QEFCA8 capable of ferulic acid biotransformation to vanillin were constructed using homologous recombination and a PCR based cloning strategy, respectively. In the liquid culture of P. putida BO14, 26.81±2.30 μg vanillin ml −1 of culture filtrate was detected. In the case of recombinant E. coli QEFCA8, 19.37±1.95 μg vanillin ml −1 of culture filtrate was detected. Results indicate that the strains could be useful for the biotechnological production of vanillin, a very important flavoring substance.

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