The S. cerevisiae Mag1 3-methyladenine DNA glycosylase modulates susceptibility to homologous recombination
DNA Repair, ISSN: 1568-7864, Vol: 1, Issue: 8, Page: 645-659
2002
- 28Citations
- 17Captures
Metric Options: Counts1 Year3 YearSelecting the 1-year or 3-year option will change the metrics count to percentiles, illustrating how an article or review compares to other articles or reviews within the selected time period in the same journal. Selecting the 1-year option compares the metrics against other articles/reviews that were also published in the same calendar year. Selecting the 3-year option compares the metrics against other articles/reviews that were also published in the same calendar year plus the two years prior.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations28
- Citation Indexes28
- 28
- CrossRef19
- Captures17
- Readers17
- 17
Article Description
DNA glycosylases, such as the Mag1 3-methyladenine (3MeA) DNA glycosylase, initiate the base excision repair (BER) pathway by removing damaged bases to create abasic apurinic/apyrimidinic (AP) sites that are subsequently repaired by downstream BER enzymes. Although unrepaired base damage may be mutagenic or recombinogenic, BER intermediates (e.g. AP sites and strand breaks) may also be problematic. To investigate the molecular basis for methylation-induced homologous recombination events in Saccharomyces cerevisiae, spontaneous and methylation-induced recombination were studied in strains with varied MAG1 expression levels. We show that cells lacking Mag1 have increased susceptibility to methylation-induced recombination, and that disruption of nucleotide excision repair (NER; rad4 ) in mag1 cells increases cellular susceptibility to these events. Furthermore, expression of Escherichia coli Tag 3MeA DNA glycosylase suppresses recombination events, providing strong evidence that unrepaired 3MeA lesions induce recombination. Disruption of REV3 (required for polymerase ζ (Pol ζ)) in mag1 rad4 cells causes increased susceptibility to methylation-induced toxicity and recombination, suggesting that Pol ζ can replicate past 3MeAs. However, at subtoxic levels of methylation damage, disruption of REV3 suppresses methylation-induced recombination, indicating that the effects of Pol ζ on recombination are highly dose-dependent. We also show that overproduction of Mag1 can increase the levels of spontaneous recombination, presumably due to increased levels of BER intermediates. However, additional APN1 endonuclease expression or disruption of REV3 does not affect MAG1 -induced recombination, suggesting that downstream BER intermediates (e.g. single strand breaks) are responsible for MAG1 -induced recombination, rather than uncleaved AP sites. Thus, too little Mag1 sensitizes cells to methylation-induced recombination, while too much Mag1 can put cells at risk of recombination induced by single strand breaks formed during BER.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S1568786402000721; http://dx.doi.org/10.1016/s1568-7864(02)00072-1; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0037031214&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/12509287; https://linkinghub.elsevier.com/retrieve/pii/S1568786402000721; http://linkinghub.elsevier.com/retrieve/pii/S1568786402000721; http://api.elsevier.com/content/article/PII:S1568786402000721?httpAccept=text/xml; http://api.elsevier.com/content/article/PII:S1568786402000721?httpAccept=text/plain; http://dx.doi.org/10.1016/s1568-7864%2802%2900072-1; https://dx.doi.org/10.1016/s1568-7864%2802%2900072-1
Elsevier BV
Provide Feedback
Have ideas for a new metric? Would you like to see something else here?Let us know