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Precise regulation of the substrate selectivity of Baeyer-Villiger monooxygenase to minimize overoxidation of prazole sulfoxides

Chinese Journal of Catalysis, ISSN: 1872-2067, Vol: 51, Page: 157-167
2023
  • 3
    Citations
  • 0
    Usage
  • 1
    Captures
  • 1
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    3
    • Citation Indexes
      3
  • Captures
    1
  • Mentions
    1
    • News Mentions
      1
      • News
        1

Most Recent News

Researchers at East China University of Science and Technology Report New Data on Chemicals and Chemistry (Precise Regulation of the Substrate Selectivity of Baeyer-villiger Monooxygenase To Minimize Overoxidation of Prazole Sulfoxides)

2024 JAN 25 (NewsRx) -- By a News Reporter-Staff News Editor at Chemicals & Chemistry Daily Daily -- Investigators publish new report on Chemicals and

Article Description

Baeyer-Villiger monooxygenases (BVMOs) can catalyze the asymmetric oxidation of sulfides to valuable chiral sulfoxides, but the overoxidation of sulfoxides to undesired sulfones limits the synthetic application of BVMOs. This overoxidation is caused by insufficient substrate selectivity of BVMOs, where the desired product sulfoxide can be further oxidized. In this study, a mathematical model was constructed to quantitatively define the substrate selectivity based on the ratio of the specificity constant ( k cat / K m ) between sulfide and sulfoxide. The substrate selectivity of a pyrmetazole monooxygenase ( Ac PSMO) was precisely regulated using a structure-guided substrate tunnel engineering approach, which successfully minimized sulfoxide overoxidation. The sulfone content of variant F277L was less than 1% (mol/mol), compared with 65% for the wild-type, in the pyrmetazole oxidation reaction after 24 h. Molecular dynamics simulations and quantum mechanics/molecular mechanics studies showed that the altered H-bonding networks surrounding the flavin hydroperoxide (FADH-OOH) can modulate the mechanism and activity for sulfoxide oxidation. Furthermore, the redesigned mutants of Ac PSMO were successfully applied for the controllable synthesis of other chiral prazole sulfoxides.

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