Fluoride exposure inhibits protein expression and enzyme activity in the lung-stage larvae of Ascaris suum
Parasitology, ISSN: 0031-1820, Vol: 133, Issue: 4, Page: 497-508
2006
- 3Citations
- 8Captures
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Metrics Details
- Citations3
- Citation Indexes3
- CrossRef3
- Captures8
- Readers8
Article Description
Sodium fluoride (NaF) is an anion that has been previously shown to block the moulting process of Ascaris suum larvae. This study describes moulting and development-specific protein expression profiles of A. suum lung-stage L3 (AsLL3) following NaF exposure. AsLL3s cultured in the presence or absence of NaF were prepared for protein analysis using two-dimensional (2D) electrophoresis. NaF exposure inhibited at least 22 proteins in AsLL3 compared with moulted larvae (i.e. AsLL4). A further comparison of AsLL4 with those of pre-cultured AsLL3 and NaF-exposed AsLL3 revealed 8 stage-specifically and 4 over-expressed proteins. Immunoblot analysis revealed an inhibition by NaF of 19 immunoreactive proteins. Enzyme assay and immunochemical data showed an inhibition of the moulting-specific inorganic pyrophosphatase activity by 41% and a decreased expression in NaF-treated larvae, indicating its significance in the moulting process. A protein spot associated with NaF inhibition was isolated and identified by peptide mass spectrometry and bioinformatics approaches to be a member of 3-hydroxyacyl-CoA dehydrogenase/short-chain dehydrogenase enzyme families. These results have implications for the identification of proteins specific to the moulting process as potential chemotherapeutic targets. © 2006 Cambridge University Press.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=33750217971&origin=inward; http://dx.doi.org/10.1017/s0031182006000576; http://www.ncbi.nlm.nih.gov/pubmed/16959050; http://www.journals.cambridge.org/abstract_S0031182006000576; https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0031182006000576; https://www.cambridge.org/core/product/identifier/S0031182006000576/type/journal_article
Cambridge University Press (CUP)
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