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Ultratrace enrichment of tyrosine phosphorylated peptides on an imprinted polymer

Analytical Chemistry, ISSN: 0003-2700, Vol: 83, Issue: 5, Page: 1862-1865
2011
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Article Description

Novel molecularly imprinted polymers (MIPs) designed to bind the side chain of phosphotyrosine can be used as artificial receptors for affinity-based enrichment of proteolytic peptides. In comparison with general enrichment methods for phosphorylated peptides such as TiO-based methods, the pTyr-imprinted polymers offered high selectivity for pTyr-containing peptides down to the low fmol level. This suggests MIPs as a new tool for affinity-based proteomics. © 2011 American Chemical Society.

Bibliographic Details

Helling, Stefan; Shinde, Sudhirkumar; Brosseron, Frederic; Schnabel, Anke; Müller, Thorsten; Meyer, Helmut E; Marcus, Katrin; Sellergren, Börje

American Chemical Society (ACS)

Chemistry

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