Microfluidic-CFPA Chip for the Point-of-Care Detection of African Swine Fever Virus with a Median Time to Threshold in about 10 min
ACS Sensors, ISSN: 2379-3694, Vol: 4, Issue: 11, Page: 3066-3071
2019
- 37Citations
- 48Captures
- 1Mentions
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations37
- Citation Indexes36
- 36
- CrossRef29
- Policy Citations1
- Policy Citation1
- Captures48
- Readers48
- 47
- Mentions1
- News Mentions1
- News1
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Article Description
The outbreak of African swine fever has brought serious public safety problems to the world, and there is a need for reliable diagnostic tools to prevent and control the epidemic. Here, we successfully established a portable microfluidic-circular fluorescent probe-mediated isothermal nucleic acid amplification (CFPA) system for the rapid, high-throughput, accurate, and sensitive detection of African swine fever virus at point-of-care settings. This detection system has a detection limit of 10 copies/μL, good stability (C.V. < 5%), and 92.73% sensitivity, and 100% specificity when tested on 220 pig samples collected for the diagnosis of African swine fever virus, with a median time to threshold of 10.8 min. This novel integrated diagnostic tool is urgently needed and has promising applications for the monitoring and control of African swine fever epidemics worldwide and, in particular, the serious outbreak in China.
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