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A P450 metabolism experiment for undergraduate biochemistry laboratories

Journal of Chemical Education, ISSN: 0021-9584, Vol: 91, Issue: 1, Page: 141-144
2014
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Article Description

A laboratory experiment is described to provide students hands-on experience in learning some aspects of microsomal P450-catalyzed metabolism. Undergraduate students in the biochemistry laboratories detect and quantify the metabolites produced from butylated hydroxytoluene (BHT) by liver microsomes using gas chromatography-mass spectrometry (GC-MS) techniques. The laboratory provides training to students for handling active microsomes, sample cleanup of biological matrices, extraction of organic metabolites, GC-MS analysis, data interpretation of complex mixtures, and collaborative work. © 2013 The American Chemical Society and Division of Chemical Education, Inc.

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